Extraction of Corosolic Acid Using CO2 Supercritical Fluid Extractor From Lagerstroemia Speciosa leaves: An Optimization and Enzyme Inhibitory Kinetics Study

Abstract

The purpose of this work was to create a rapid and effective supercritical fluid extraction (SFE) method for removing corosolic acid (CA) from Lagerstroemia speciosa (LS). Using a quadratic polynomial model, response surface methodology (RSM) was used to optimize the process. The independent variables chosen for this study were the extraction time, pressure, temperature, and ethanol content of the modifier. Response surface plots showed that the four independent variables interacted to affect the CA's yield. The results showed that 62.77 minutes of extraction time, 24.005 MPa of pressure, 58.145ºC of temperature, and 71.624% of modifier were required to produce the maximum total yield. When the ideal circumstances were followed, the experimental yield of 8.20 mg/g was very close to the figure that the model had anticipated. Based on the kinetics of inhibition, a novel role of CA as an α-glucosidase inhibitor was investigated. CA α-glucosidase inhibitory action (IC50) was 1.85×10⁻⁵ mol L⁻¹, indicating an uncompetitive inhibition mechanism. The results of this study shed light on the possible development of CA as an α-glucosidase inhibitor in certain dietary items.