Comparative Assessment of Anti Microbial Efficacy of Curcumin Loaded Different Nanoparticle Coated Gutta Percha- In Vitro Study

Abstract

Background: By encasing any remaining bacteria within the root canal and serving as a barrier to external microbes, GP fillings prevent infection. Adding more materials to their composition may be one way to boost their antibacterial action.

Aim: to evaluate the antibacterial activity of guttapercha-coated with various nanoparticles loaded with curcumin.

Material and methods: Forty GP cones were obtained, according to the coating of nanoparticles and nanocurcumin, they were allocated into Group 1 - Conventional GP; Group 2 - Nanocurcumin coated GP; Group 3 – Nano curcumin loaded chitosan coated GP; Group 4 – Nano curcumin loaded titanium dioxide coated GP. The bacterial strain used in this study was E. faecalis ATCC 29212. The antibacterial activity was determined using the agar diffusion technique. The surface topography of the coated and untreated GP cones was evaluated using SEM.

Results: A significant difference was found between the groups, with Group 3 exhibiting the highest zone of inhibition, followed by Group 4 and Group 2. Group 1 had the smallest zone of inhibition. Post hoc analysis further confirmed significant differences between the groups. The uncoated GP surface showed noticeable unevenness and irregularities to the coated cones.

Conclusion:  Nanocurcumin had a significant antibacterial effect against the E. faecalis pathogen. Further coating of chitosan and titanium dioxide to nanocurcumin-loaded GP produced a synergistic antibacterial effect against the pathogen.