Graphical impression for siderophore production by Vibrio campbelli using Central composite design

Abstract

Background: This study focused on enhancing siderophore production by Vibrio Campbellii (NIOT/SID/43) through optimization of growth conditions using response surface methodology (RSM).

Methods: Vibrio Campbellii (NIOT SID 43) initial screening identified MM9 medium as optimal, with parameters set at 28°C, pH 5.5, 0.4% glucose, and 10 μmol Fe (III).

Result : Quantitative and qualitative siderophore assays using the CAS assay confirmed maximum production at these conditions (95.64%). Subsequent optimization using central composite design within RSM identified key factors influencing production: temperature (37°C), pH (8.75), glucose concentration (0.50%), and iron availability (25 μmol Fe (III)). Statistical analysis yielded a significant quadratic effect (F-value 95.19, R² = 0.9943), indicating robust model validity. This optimized approach resulted in a substantial increase in siderophore yield, demonstrating the efficacy of RSM in enhancing bioprocess efficiency. These findings provide insights into improving siderophore production for potential biotechnological applications in iron-challenged environments.