Development and Validation of RP-HPLC Method for Quantification of Quercetin in Solanum nigrum Extract

Abstract

Solanum nigrum is thought to contain and demonstrate a wide range of biological activities, including anticancer, antioxidant, antiulcerogenic, and antipyretic properties. Several analytical techniques have been documented for the quantification of Quercetin. Quercetin, a flavanol, found in a variety of plants. A recent discovery in analytical chemistry offers a practical, affordable, and responsible way to build different analytical techniques. Development of RP-HPLC method using for the flavonoid (Quercetin) in the hydroalcoholic extract of Solanum nigrum Linn. Establishment of chromatographic method involved employee RP-HPLC method to ensure robust, precise and accurate quantification of quercetin. Acetonitrile: High-quality water in an isocratic elution mode, at a flow rate of 1 mL/min at a temperature of 37±2⁰C. The approach was proven to be specific for analysis of quercetin which was accurate (>90%) and precise (%RSD <2%). A calibration curve was reputable over the quercetin concentration range, with a correlation value of 0.9992. The LOD was 22.45µg and LOQ value was found to be 68.05µg. The RSD of the proposed approach was determined by assaying six replicate quercetin injections intra-day and inter-day. The average quercetin concentration of Solanum nigrum Linn. was determined to be 6.317 mg/kg. The method was new prepared and validated with the parameters according to guidelines.