Molecular Genetic Study to Reveal the Association Between RYR3 Gene Expression and Gender Dysphoria

Abstract

Gender dysphoria may have a genetic component, but association study data are conflicting. Brain development diverges in males and females in response to androgen production by the foetal testis. This sexual differentiation of the brain occurs during a sensitive window and induces enduring neuroanatomical and physiological changes that profoundly impact behavior. We hypothesised that gender is an innate part of identity, and we hypothesised that RYR3 would be a part of individuals’ transsexuality. By utilising quantitative real-time PCR (RT-PCR) gene expression, we analysed the RYR3 expression level in 50 transgender blood samples. The gene expression level of RYR3 was analysed statistically. A significant association was identified between gender dysphoria and RYR3 expression level. A significant difference in the gene expression level of RYR3 was observed in various age groups. The gene expression ratio was observed in the age groups of 17–20 (0.2826), 20–29 (0.5468), 30-39 (0.2425), 40–49 (0.2006), and control patients (1.000). Our result proposed that there is a low level of expression observed in all the groups when compared to the control group.