Determent's of some virulence genes in Uropathogenic Escherichia coli

Abstract

In order to isolate and identify uropathogenic E. coli, 199 urine specimens from patients with urinary tract infections (UTIs) at Sadder Medical City and Al-Sajjad hospital in the Najaf governorate were collected between March and June 2022. The specimens were then cultivated on media such as MacConkey, blood agar, Nutrient broth mixed with glycerol, and EMB agar. The primary identification process relied on colonial structure, microscopic study, and biochemical tests, while the final identification was done using the automated VITEK-2 compact system with Gram negative identification cards (GN-ID). In this investigation, the prevalence of UTI was higher in women (39.13%) than in men (17.85%), and only 60 (30.15%) of the isolates from the 199 urine samples were linked to E. coli. Sample 45 (female) had the greatest incidence, followed by sample 15 (male). The study looked into the virulence factors that lead to the pathogenesis of Escherichia coli. Of the isolates, 25.12% were able to produce haemolysin, 70.61% were able to form biofilm, and all isolates were able to form capsules. However, none of the isolates were able to produce gelatinase. The study examined the antibiotic sensitivity of 35 isolates. The findings indicate that the isolates exhibited resistance to Ciprofloxacin, Gentamicin, Ceftazidime, Trimethoprim-Sulfamethoxazole, 68.59%, 45.55%, 38.71%, and 10.16%, respectively, and that 100% of the E. Coli isolates were susceptible to Amikacin and Imipenem. Ultimately, afaC, the hlyCA area, cnf, pap, and fim A genes were found utilizing the molecular approach and the Polymerase Chain Reaction (PCR) technology. The findings revealed that 69% of E. coli had the fim A gene, whereas 85% of E. coli carry the pap gene, 77% carry the afaC gene, 66% the hlyCA area, and 55% the afaC gene.