Ameliorative effect of acetaminophen-agmatine sulphate co administration on induced fever, pain and inflammation in male rats

Abstract

The main aim of this study is to ameliorate the therapeutic profile namely; antipyretic, analgesic and anti-inflammatory effects of acetaminophen by using different doses of acetaminophen-agmatine sulphate combinations. Forty male Wister albino rats, by a middling weight of 225 grams, was used in this study and divided into five groups for every experiment; Group(G1) treated with dosage of agmatine sulphate alone 1000mg/225g BW, orally. Group(G2) treated with dosage of acetaminophen alone 1000mg/225g BW, orally. Group(G3) treated with co-administration of acetaminophen with dosage 90% 0f 1000mg/225g BW and agmatine sulphate with dosage 10% of 1000mg/225g BW, respectively orally. Group(G4) treated with a co-administration acetaminophen with dosage 70% of 1000mg/225g BW and agmatine sulphate with dosage 30% of 1000mg/225g BW, respectively orally. Group(G5) treated with co-administration acetaminophen with dosage 50% of 1000 mg/225g BW and agmatine sulphate with dosage 50% of 1000mg/225g BW, respectively orally. The results showed in hyper thermic experiment all groups treatment there are no significant value (P<0.05) between them to decreasing the temperature. While, group 2,4 and there decrease significant (P<0.05) in the level of prostaglandin compering with group 1,3 and induction group.  Moreover, the nociceptive response in the formalin test, for acute pain, in phase 1(5mint) group1 had decrees significant (P<0.05) in number of flinching and licking compared with other treated groups and induction group. While, in phase 2 (20mint) group1,3 had decrees significant (P<0.05) in number of flinching and licking compared with other treated groups and induction group. In writhing test group1,4 had decrease significant (P<0.05) for incidence of writhing by acetic acid, associated by visceral pain. the outcomes showed a reduction significantly (P<0.05) of paw thickness and white blood cells in group1 and minor infiltration of inflammatory cells showed in group1 of histopathological of paw compared with other groups. The study concluded that in co-administration acetaminophen and agmatine sulphate in any dosage had anti hyper thermic effect and increase in the dosage of agmatine sulphate combined with paracetamol resulted in enhanced analgesic efficacy. Finally, Agmatine ameliorate the therapeutic profile of acetaminophen as weak anti-inflammatory.