Molecular Detection of Carbapenem resistant Klebsiella Pneumoniae at a tertiary Care hospital Indore, India.
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Keywords

Carba NP test
modified Carbapenem Inactivation Method (mCIM)
Real time polymerase chain reaction (rt-PCR)
Carbapenem Resistant Enterobacteriaceae (CRE)

Abstract

Background: Klebsiella pneumoniae isolates, resistant to carbapenem drugs is commonly transmitted by hospital settings. This is due to infections which lead to mortality and morbidity. Therefore, the study on molecular level will help to gain more information and be able to know their cross-transmission and resistance mechanisms among these isolates showing resistance. Aim and objective: To detection of carbapenem resistant genes of klebsiella pneumoniae isolates from different clinical sample by RT-PCR. Klebsiella pneumoniae are aerobic and Gram-negative bacilli found in the gastrointestinal tract of humans. Method: In this study, 156 isolate of klebsiella pneumoniae and Escherichia coli which is resistant to carbapepenem by phenotypic methods. Result: 60 isolates of klebsiella pneumoniae were confirmed by carbaNP and mCIM to resistant carbapenem drugs and also confirmed by real time polymerase chain reaction for various genes. Conclusion: This current study concluded the detection of carbapenem resistant genes of klebsiella pneumoniae could help the find out the route of dissemination of species and may control its spread within the hospital settings.

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