Ecofriendly green synthesis and characterization of silver-zinc oxide nanocomposite using fresh leaf extract Evolvulusalsinoides and evaluate their antibacterial and anticancer activity

Abstract

In materials science, the method of green synthesis is rapid, reproducible, sustainable and eco friendly in nature. It has wide range of nanomaterials which includes metal, metal oxide, hybrid and bio inspired materials. In this study, green synthesis of silver- zinc oxide nanocomposites using fresh leaf extract Evolvulus alsinoides by co-precipitation method. The characterization studies are done by X-Ray diffraction analysis (XRD), Fourier Transform Infrared spectral analysis (FTIR), UV-Visible spectral analysis (UV-Vis), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray analysis (EDX), Antibacterial and Anticancer activity. In XRD analysis, the particle size is reduced by increasing concentration of silver. The functional groups and chemical bonds of ZnO and Ag- ZnO NCs via the leaf extract Evolvulus alsinoides are investigated using FTIR spectroscopy. The absorption wavelength is increased and bandgap energy is decreased for synthesized ZnO and Ag-ZnO NCs. The morphological analysis of ZnO and Ag-ZnO NCs are determined by Scanning Electron Microscopy. The elemental composition of ZnO and Ag-ZnO NCs are confirmed by EDX analysis. The zone of inhibition against gram positive and gram-negative bacteria is determined by antibacterial activity using agar well diffusion and results the decrease in inhibition zone by silver content. The anticancer activity has good efficacy for ZnO NPs against HT-29 cell line.