Fertility Booster Effect of Selaginella Bryopteris (Sanjivani) Against High Dose of Estradiol Exposed Swiss Albino Mice
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Keywords

Estradiol
Selaginella bryopteris
LH
FSH
Testosterone
and Mice

Abstract

The present investigation was aimed to examine the antifertility effects of estradiol and phytochemical influences of Selaginella bryopteris (Sanjivani) in Swiss albino mice. Male twentyfour (n=24) mice were selected which was divided into four different group (n=6; each). Group-1 (G1): control, Group-2 (G2): estradiol treated, Group-3 (G3): Pre-treated estradiol + Selaginella bryopteris (150mg/kg body weight) and Group-4 (G4): Pre-treated estradiol + Selaginella bryopteris (200mg/kg body weight).  Forty-five days of estradiol treatment at 25μg/kg body weight were given to G2, G3, and G4 mice. After completion of dose duration G2 were stopped for examination and the rest G3 and G4 were continued for Selaginella bryopteris administration at two different doses for 35 days. Sperm quality, immunoreactive luteinizing hormone (LH), follicle stimulating hormone (FSH), and testosterone (T) were analysed. The outcomes of this study indicated that compared to G1 the estradiol treated G2 had significant (p<0.001) alteration in sperm count, sperm motility, sperm morphology, serum level of LH, FSH, and testosterone. Rebound effects were observed in G3 and G4 after the administration of S. bryopteris. Compared to G3 vs. G4; the G4 had better results than G3. The testicular architecture was analysed through histological study of testis revealed disorganization of the cytoarchitecture in the seminiferous tubules, vacuolations, absence of lumen and compartmentalization of spermatogenesis. Compared with LH and sperm density, estradiol significantly suppressed FSH and sperm motility. It is evident that the direct action of estradiol on the testis is mostly responsible for the T reduction. But, the administration of S. bryopteris 200 mg/kg body weight had resolve the alteration bitterly than 150 mg/kg body weight of S. bryopteris.

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