The Anti Cancer Activity Of Purified Tannins Extracted From Blackcurrant Flower On The Proliferation And The Migration Of Cancer Cells

Abstract

While the growing interest in natural compounds for their health beneficial properties. One such natural compound that has gained attention is purified tannins. These tannins have shown promising activity in various studies. This study was conducted to examine the effect of purified condensed tannins (CTs) extracted from blackcurrant flowers against the proliferation and migration of cancer cells. Purified CT extracts (up to 90%) were prepared from petals of blackcurrant (Ribes nigrum) flowers that were gained with the Sephadex LH20 column. The analytical thiolysis, HPLC and Mass spectrometry (MS) assays were also conducted to determine CT contents, including compositions and concentrations. Serial dilutions (4mg/ml, 2mg/ml and 1mg/ml) of the purified CT extracts were prepared to test their effects against the proliferation and migration of breast cancer cells (MDA-MB-231). The results revealed the maximum effects accompanied by the concentrations of CTs (4mg/ml and 2mg/ml) which have a cytotoxic effect on cancer cells. The lowest concentration (1mg/ml) delayed the cell doubling time 3-4 hours. Wound healing assay showed a significant reduction in wound closer of treated cells (1mg/ml) in comparison with control cells. These results suggested anticancer effects of CTs in vitro which also have inhibitory effects against the proliferation and migration activity of breast cancer cells.