Derivative Spectroscopy Application In Simultaneous Quantification Of Anti – Viral Drugs

Abstract

Simple, precise and accurate methods for simultaneous quantification of sofosbuvir and daclatasvir in combined tablet dosage forms using derivative spectroscopy have been developed. The methods were based on ratio derivative and first derivative method using 0.1 N HCl as solvent, For ratio derivative and first derivative the absorbance were measured at selected wavelength which are 246 nm for sofosbuvir and 324 nm for Daclatasvir in combined formulation. Beers law is obeyed in the concentration of 5-30 µg/ml for Sofosbuvir and 2-12 µg/ml for Daclatasvir. The % assay in commercial formulation was found to be 99.6 for Sofosbuvir and 100.4 for daclatasvir for first derivative spectroscopy and 97.45 for sofosbuvir and 99.71 for daclatasvir for ratio derivative spectroscopy. The methods were validated with respect to linearity, precision, accuracy, recovery was found in the range of 95-101 % for sofosbuvir and 96-103% for daclatasvir for first derivative method and 97-101 % for sofosbuvir and 98- 102% for sofosbuvir for daclatasvir for ratio derivative method respectively for formulation. The methods developed were simple, economical, precise and accurate can be used for routine quality control of combined tablets.