Study On In Vitro Propagation Of Dioscorea Collettii Hook.F.

Abstract

The study aimed to develop a rapid multiplication process for Dioscorea collettii Hook.f. 1892 using the in vitro culture method. The optimal sterilization procedure involved shaking the explants in a 20% NaClO solution for 25 minutes, followed by two rounds of shaking in a 10% H2O2 solution for 10 and 5 minutes. The explants were then cultured on a basic MS medium without plant growth regulators and observed for 15 days, resulting in a 90.33% survival rate. The MS medium was supplemented with a 2.0 mg/l concentration of the BAP regulator to promote shoot regeneration from stem segments, which resulted in a 93.33% regeneration rate. The highest multiplication factor of 8.72 was achieved using the MS medium supplemented with 2.0 mg/l BAP and 0.4 mg/l NAA. The most effective medium for root induction and complete plant formation was ½ MS medium supplemented with 0.5 mg/l activated carbon and 1.0 mg/l NAA. This medium yielded a 100% root induction rate and an average of 5.63 roots per shoot. After 6 weeks of culture, seedlings with fully developed roots were transferred from the culture vase for in vivo growth.