Characterization And Development Of Pegylated Nanoparticles Loaded With Thujone For Improved Brain Targeting

Abstract

The objective of this study was to develop biodegradable PEGylated chitosan nanoparticles and evaluate their potential for targeted delivery to specific areas of the brain on a single platform. We created and tested a PEGylated chitosan nanoconjugate designed to transport Thujone, a natural anti-Alzheimer’s agent, to the brain. Utilizing the ionic gelation method, we synthesized the Thujone-loaded nanoparticles (TPNs) and assessed various optical and in vitro parameters. The MTT assay performed on UCSD229i-SAD1-1 human astrocytoma cells indicated IC50 values of 0.42, 0.49, 0.67, and 0.75 µM for the Thujone-loaded PEGylated nano system. Enhanced uptake and biodistribution studies, conducted via confocal microscopy and apoptosis assays, confirmed a significantly greater accumulation of the nanoconjugate in the brain compared to the free Thujone solution, as validated by in vitro evaluations of cell lines. Notably, the targeting potential of TPNs was found to be twice as effective as that of the free Thujone solution. In conclusion, the biodegradable PEGylated chitosan nanoconjugate shows promise as a nano-targeting platform to improve the delivery of anti-Alzheimer medications to the brain, potentially leading to better therapeutic outcomes.Top of FormBottom of Form