Formulation Development And Characterization Of Pegylated Self Nano Emulsifying Formulation Containing Gentamicin

Abstract

Self-nanoemulsifying formulations (SNEFs), enhanced with PEG 4000 to create PEGylated SNEFs, were developed for the oral delivery of gentamicin. These formulations were designed following solubility tests, using a blend of soybean oil, Kolliphor1 EL and Kolliphor1 P188 as surfactants, and Transcutol1 HP as a co-surfactant. The incorporation of gentamicin and PEG 4000 decreased the initial nanoemulsion area in ternary phase diagrams created by water titration with oil, surfactants, and co-surfactant. The resulting emulsion droplets were nano-sized, between 80-210 nm. FT-IR analysis confirmed that gentamicin's structure remained unchanged across all formulations, and SEM images displayed spherical globules. Zeta potential measurements ranged from -25.4 to -42.5 mV, indicating a stable system with minor electrostatic fluctuations. The SNEFs demonstrated high in vitro diffusion-dependent permeation of gentamicin. This study concluded that formulating gentamicin as surface-modified SNEFs significantly enhanced its oral delivery.