Neurorestorative Potential of Persea Americana Fruits Extract In Experimental Animals

Abstract

Background & Objectives: The present investigation was intended to explore the Neurorestorative effect of methanolic extract of fruits of Persia americana at various dose levels (125 mg/kg, 250 mg/kg and 500 mg/kg) using different animal models.

Methods: In present research work, anoxia stress tolerance test, immobilization stress are considered to evaluate the anti-stress effect and elevated plus maze test, open field apparatus test were used to evaluate the anxiolytic effect of methanolic extract of Persia americana fruits in Wistar rats and Swiss albino mice.

Results: The MEPAF at various doses i.e. 125, 250 and 500 mg/kg of the test extract produced a dose dependent significant delay in convulsions on 7^th, 14^th and 21^st day compared to control group. The treatment with various doses of MEPAF and standard drug Diazepam significantly attenuated the altered levels of hematological parameters such as RBC, WBC and Platelets count when compared to stress control animals. The MEPAF administered animals for ten days at various doses exhibited significant and dose dependent decrease in serum biochemicals as compared to the stress control rats. Pretreatment of various doses of MEPAF for ten days, significantly and dose dependently attenuated the immobilization stress induced altered organs weight. The graded doses of the MEPAF and diazepam exhibited significant increase in number of entries into the open arm and time spent in the open arm whereas decrease in the number of entries into the closed arm and time spent in closed arm compared control group. The results of this experimental model showed that the test extract at graded doses significantly increased ambulation, rearing and significant decrease in self-grooming and fecal dropping in dose dependent manner as compared to the control group.

Conclusion: In conclusion, the methanolic extract of Persea americana exhibited dose dependent significant anti-stress and anxiolytic activity in experimental models of stress and anxiety. Thus the methanolic extract Persea americana fruits serves as an anti-stress and anti-anxiety in the management of stress and stress related problems.