Identification and antibiotic susceptibility pattern of bacteria isolated in Laboratory (ART)

Abstract

Infertility, affecting millions worldwide, is driven by genetic, environmental, infectious, and lifestyle factors. The growing use of Assisted Reproductive Technologies (ART) like IVF helps address infertility challenges. The present study investigated bacterial contamination in semen samples of males undergoing fertility assessments in ART labs, revealing the presence of bacteria such as Escherichia coli  (E. coli), Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa (P. aeruginosa), and Staphylococcus aureus (S. aureus). These findings emphasize the need for enhanced sterility protocols and an understanding of antibiotic resistance patterns. Notably, E. coli obtained in the study exhibited high resistance to Gentamycin (71.92%) and Ceftriaxone (74.35%), S. aureus displayed substantial resistance to Ciprofloxacin (79%), K.pneumoniae demonstrated significant resistance across most antibiotics, notably to Ceftriaxone (79.31%) and Gentamycin (75.86%), and indicating critical challenges in treatment, P. aeruginosa presented a mixed resistance pattern, with Pipracillin with Tazobactam. The result underscores the importance of regular susceptibility testing to guide treatment and improve ART success rates.