Evaluation of Antimicrobial Efficacy of New Bio-C Root Repair Material in Comparison to Biodentine, MTA, and Glass Ionomer Cement Against Enterococcus Faecalis-An In Vitro study

Abstract

Background: Perforation is an artificial communication between the oral environment and supporting structures that occurs during access cavity preparation, post-surgery, or as a result of internal resorption extending into periradicular tissues. With a prevalence of 88.7%, enterococcus faecalis is one of the persistent microorganisms that are isolated from the pathological sites in cases of persistent infection. Therefore, the use of root repair materials with antibacterial activity is considered beneficial. Aim: Evaluation of antimicrobial efficacy of new Bio-c Root Repair Material in comparison to Biodentine, MTA, and Glass Ionomer Cement against Enterococcus Faecalis-An in vitro study. Materials and Methods The antimicrobial activity of root repair materials was assessed using agar well diffusion method.  It was divided into 4 groups Group I: Bio-C, Group II: Biodentine, Group III: MTA, Group IV: GIC. The plates were then incubated at 37°C for 24, 48 and 72 hours. After incubation, the diameter of inhibition zones were measured in millimeters using Antibiotic Zone Scale (Himedia, Mumbai) at 24, 48 and 72 hour. The results obtained were subjected to statistical analysis by one way ANOVA and Tukey Post Hoc test. Results: At 24 hours the antimicrobial efficacy of root repair materials against E. faecalis in different groups was: Group II > Group III > Group I > Group IV At 48 hours the antimicrobial efficacy of root repair materials against  E. faecalis in different groups was: Group II > Group III = Group I > Group IV. At 72 hours the antimicrobial efficacy of root repair materials against  E. faecalis in different groups was:Group II > Group III = Group I > Group IV. Conclusion: Based on the result obtained, it was concluded that Biodentine showed highest antimicrobial efficacy at 24, 48, and 72 hours than all other groups.