Microwave-Assisted Green Synthesis Of Mno2 Nanoparticles Using Aqueous Leaf Extract Of Solanum Nigrum And Its Anti-Microbial And Antibiofilm Applications

Abstract

The green synthesis of metal oxide nanoparticles provides an alternative method to chemical synthesis. The green synthesized metal nanoparticles possess a high surface area, good biocompatibility, and good mechanical stability, and possess a wide range of uses in the fields of biomedicine, photocatalysis, sensing, antibacterial action, and antibiofilm activity. A biofilm is a group of bacteria attached to living or non-living surfaces that are relatively resistant to antimicrobial agents causing persistent infections. Nanotechnology including metallic nanoparticles, polymeric nanoparticles, and functionalized metallic nanoparticles has provided novel solutions for preventing biofilm formation.  FTIR, UV, SEM, EDX, and XRD examined the synthesized MnO₂ nanoparticles in this study. The average crystalline size of the synthesized nanoparticles was 17.78 nm and was used to evaluate antibiofilm and antimicrobial activities. MnO₂NPs were researched for their ability to inhibit S. aureus and E.coli biofilms. MnO₂ NPs at a concentration of 150 μg/mL resulted in a high inhibition value of approximately 98% for S. aureus biofilm formation and E.coli's inhibition value was approximately 99%. Therefore, the synthesized MnO₂ NPs could be used in many applications because of their antimicrobial and antibiofilm properties.